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| Registros recuperados: 76 | |
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| Garcia,Edivaldo E; Kimura,Cláudia C.M; Martins,Ariovaldo C; Rocha,Gisele O; Nozaki,Jorge. |
| Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chrome shavings; Enzyme; Chromatography; Protein; Chromium. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003 |
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| Teixeira,Maria F. S.; Lima Filho,José L.; Durán,Nelson. |
| The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectinases; Aspergillus japonicus; Enzyme; Galacturonase; Pectinesterases. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400009 |
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| Aidar,Davi S.; Pagotto,Rubiane C.; Contel,Eucleia P.B.. |
| Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melipona quadrifasciata anthidioides; Polymorphism; Electrophoresis; Enzyme; Stingless bees. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018 |
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| Facincani,Agda Paula; Ferro,Jesus Aparecido; Pizauro Jr.,João Martins; Pereira Jr.,Haroldo Alves; Lemos,Eliana Gertrudes de Macedo; Prado,Alessandro Luis do; Ferro,Maria Inês T.. |
| The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor), Triton X-100, and TCA.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enolase; Entner-Doudoroff; Enzyme; Glycolysis; Xylella fastidiosa. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200015 |
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| Silva,Hunaldo Oliveira; Fialho,Elias Tadeu; Freitas,Rilke Tadeu Fonseca de; Lima,José Augusto de Freitas; Logato,Priscila Rosa Vieira; Schoulten,Neudi Artemio. |
| It was intended with the present work to verify the effect of phytase on the performance, bone mineralization and content of plasma urea of swine. 80 swine (30 ± 3.1 kg/LW), crossbred (LD x LW) males and females, allocated to a randomized block design (RBD) with four treatments and five replicates. The treatments consisted of a growing swine diet on the basis of corn, soybean meal and defatted rice bran (DRB) supplemented with four levels of phytase (0, 400, 800 and 1200 FTU/kg) The diet was formulated to meet the requirements of growing swine except for available calcium and phosphorus, the levels of which were 0.64 and 0.25%, respectively. The enzyme phytase utilized was Natuphos 5000. Over the experimental period every eight days, blood of two animals... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme; Minerals; Plasma; Urea. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600028 |
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| Brandelli,Adriano; Riffel,Alessandro. |
| The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Enzyme; Keratin; Protease. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007 |
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| Oliveira,GH; Berchieri Jr,A; Montassier,HJ. |
| This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008 |
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| Le Moullac, Gilles. |
| Summer mortality of the oyster result from the interaction of many factors. A respiratory assumption proposes a link between the supposed causes of the energy state, the cultivation methods and the medium. This work is focused towards the search for specific metabolic markers of environmental stresses. The cDNA of genes coding for PK and PEPCK were cloned and sequenced in order to detect their regulation level. The sediment nearness affects the state of energy reserves, the metabolic pathways of energy production implying a regulation of the PK. Face to the dissolved oxygen decline, oysters were oxyregulator but with a low regulation ability. In hypoxia, glycolysis was slowed down. PK activity was inhibited resulting from an increase in alanine sensitivity... |
| Tipo: Text |
Palavras-chave: Régulation; Enzyme; Expression génique; Anoxie; Bivalve. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/these-4315.pdf |
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| Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M. |
| To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is... |
| Tipo: Text |
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf |
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| Registros recuperados: 76 | |
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